منابع مشابه
Cellular endocytosis and gene delivery.
Endocytosis is the process by which cells take up macromolecules from the surrounding medium. The best-characterized process is the so-called clathrin-dependent endocytosis, although much is also currently known about clathrin-independent endocytic processes such as those involving caveolae and lipid rafts. An understanding of endocytosis and the cellular trafficking that occurs thereafter has ...
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Transfection efficiency was the primary goal for in vitro gene delivery mediated by nonviral gene carriers. Here, we report a modified gene transfection method that could greatly increase the efficiency of, and accelerate the process mediated by, 25 kDa branched polyethyleneimine and Lipofectamine™ 2000 in a broad range of cell strains, including tumor, normal, primary, and embryonic stem cells...
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Objective(s): Lipid-based nanoparticles (NLP) are PEGylated carriers composed of lipids and encapsulated nucleic acids with a diameter less than 100 nm. The presence of PEG in the NLP formulation improves the particle pharmacokinetic behavior. The purpose of this study was to prepare and characterize NLPs containing MDR1 siRNA and evaluate their cytotoxicity and cellular uptake. MDR1 siRNA coul...
متن کامل20 Nanotechnology for Engineering Cellular Microenvironment and Gene Delivery
Tissue engineering aims at repairing and restoring lost tissue structure and functions caused by disease processes or traumatic events (Langer and Vacanti 1993). Driven by clinical demands, extensive research efforts have been dedicated toward regenerating a broad range of tissues, from the skin, cartilage, bone, and blood vessels to more complex organ-level systems such as the bladder. Most ti...
متن کاملModified pectin-based carrier for gene delivery: cellular barriers in gene delivery course.
The use of polysaccharides as DNA carriers has high potential for gene therapy applications. Pectin is a structural plant polysaccharide heterogeneous with respect to its chemical structure. It contains branches rich in galactose residues which serve as potential ligands for membrane receptors interaction. In order to make the anionic pectin applicable for DNA complexation, it was modified with...
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ژورنال
عنوان ژورنال: Molecular Medicine
سال: 2010
ISSN: 1076-1551,1528-3658
DOI: 10.2119/molmed.2009.00101